11.5.3. Measure Signal Integrity and Setup and Hold Margin
Measure the signals on the PCB. When measuring any signal, consider the edge rate of the signal, not just its frequency. Modern FPGA devices have very fast edge rates, therefore you must use a suitable oscilloscope, probe, and grounding scheme when you measure the signals.
You can take measurements to capture the setup and hold time of key signal classes with respect to their clock or strobe. Ensure that the measured setup and hold margin is at least better than that reported in the Intel® Quartus® Prime software. A worse margin indicates a timing discrepancy somewhere in the project; however, this issue may not be the cause of your problem.
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