Description
In the Quartus® Prime Standard Edition Software, you may see this error when no transceiver is used in the design but the following entry is in the Quartus Setting File (.qsf):
set_global_assignment -name AUTO_RESERVE_CLKUSR_FOR_CALIBRATION OFF
This assignment prevents the CLKUSR from being automatically reserved.
Resolution
To avoid this error, remove this assignment from the .qsf