Due to a problem in the Quartus® Prime Pro Edition Software version 22.4 and onward, you may see no output data on o_tx_serial[1:0] pins, and o_tx_ready does not toggle when only hard reset [i_rst_n] is applied to F-Tile Ethernet FPGA Hard IP with '50GE-2' variant.
To work around this problem in the Quartus® Prime Pro Edition Software version 22.4 and onward, apply i_tx_rst_n, i_rx_rst_n and csr_rst_n as well as i_rst_n.
This problem is scheduled to be fixed in a future release of the Quartus® Prime Pro Edition Software.