2.2.1. Quartus Key File Assignment
You specify a signature chain in your Intel® Quartus® Prime software project to enable the authentication feature for that design.
From the Assignments menu, select Device > Device and Pin Options > Security > Quartus Key File, then browse to the signature chain .qky file you created to sign this design.
set_global_assignment -name QKY_FILE design0_sign_chain.qky
To generate a .sof file from a previously compiled design, that includes this setting, from the Processing menu, select Start > Start Assembler. The new output .sof file includes the assignments to enable authentication with the provided signature chain.